primary antibody against osteopontin (polyclonal rabbit)  (Spring Bioscience)

Journal: Journal of Clinical Medicine

Article Title: Circulating Levels of Osteopontin Predict Patients’ Outcome after Resection of Colorectal Liver Metastases

doi: 10.3390/jcm7110390

Figure Lengend Snippet: Osteopontin (SPP1) is overexpressed in colorectal liver metastases. ( a ) Osteopontin (SPP1) mRNA expression levels are strongly elevated in colorectal cancer (CRC) tumour samples compared to normal colon tissue samples ( u -test, p (***) < 0.001); ( b ) SPP1 mRNA expression levels are significantly elevated in colorectal liver metastases (CRLM) tissue samples compared to normal liver tissue ( u -test, p (**) < 0.01); ( c ) Immunohistochemical osteopontin staining in three exemplary CRLM samples (left, middle and right column). Left column: osteopontin is highly expressed in CRLM compared to normal liver tissue; middle and right column: tumour cells show a strong osteopontin expression (black arrow heads) while the peritumoral stromal tissue displays a weak osteopontin signal (black arrows); (upper panel: 100-fold magnification, middle panel: 200-fold magnification, lower panel: 400-fold magnification).

Article Snippet: The primary antibody against osteopontin (polyclonal rabbit, Spring Bioscience, Pleasanton, CA, USA) was incubated to the sections at RT for 30 min, diluted at 1:100.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Journal of Clinical Medicine

Article Title: Circulating Levels of Osteopontin Predict Patients’ Outcome after Resection of Colorectal Liver Metastases

doi: 10.3390/jcm7110390

Figure Lengend Snippet: Circulating levels of osteopontin are elevated in patients with CRLM. ( a ) Circulating osteopontin levels are significantly elevated in patients with CRLM compared to healthy controls ( u -test, p (***) < 0.001); ( b ) Osteopontin levels show a similar diagnostic power compared to carcinoembryonic antigen (CEA) serum levels in receiver operating characteristic (ROC) curve analysis, while serum levels of bilirubin, alkaline phosphatase (ALP) and alanine aminotransferase (ALT) are unsuitable for the differentiation between CRLM patients and healthy controls.

Article Snippet: The primary antibody against osteopontin (polyclonal rabbit, Spring Bioscience, Pleasanton, CA, USA) was incubated to the sections at RT for 30 min, diluted at 1:100.

Techniques: Diagnostic Assay

Journal: Journal of Clinical Medicine

Article Title: Circulating Levels of Osteopontin Predict Patients’ Outcome after Resection of Colorectal Liver Metastases

doi: 10.3390/jcm7110390

Figure Lengend Snippet: Elevated preoperative levels of circulating osteopontin are associated with a reduced overall survival after resection of colorectal liver metastases. ( a ) Patients with high preoperative osteopontin concentrations (>75th percentile) show a significantly impaired overall survival (OS) compared to patients with low osteopontin levels (log-rank test, p = 0.008); ( b ) Patients with initial osteopontin serum above our established ideal cut-off value (264.4 ng/mL) show a strikingly reduced median OS of 304 days compared to 1394 days for patients with osteopontin serum levels below this cut-off (log-rank test, p = 0.003).

Article Snippet: The primary antibody against osteopontin (polyclonal rabbit, Spring Bioscience, Pleasanton, CA, USA) was incubated to the sections at RT for 30 min, diluted at 1:100.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Circulating Levels of Osteopontin Predict Patients’ Outcome after Resection of Colorectal Liver Metastases

doi: 10.3390/jcm7110390

Figure Lengend Snippet: Postoperative osteopontin serum levels are associated with the patients’ prognosis. ( a ) Circulating osteopontin levels are significantly higher after tumour resection compared to preoperative levels (Wilcoxon signed-rank test, p (***) < 0.001); ( b ) Patients with postoperative osteopontin serum levels above the 75th percentile display a trend towards an impaired prognosis (log-rank test, p = 0.097); ( c ) At our ideal cut-off of 310.9 ng/mL, postoperative osteopontin concentrations significantly discriminate between patients with good or poor prognosis (log-rank test, p = 0.002); ( d ) Longitudinal changes of osteopontin serum concentrations before and after tumour resection are unsuitable to predict patients’ outcome (log-rank test, p = 0.572).

Article Snippet: The primary antibody against osteopontin (polyclonal rabbit, Spring Bioscience, Pleasanton, CA, USA) was incubated to the sections at RT for 30 min, diluted at 1:100.

Techniques: